Perfecting Your Spread Plate Technique

Perfecting Kant's highest good

Contrast Spread Technique: Evolution

The recognition of needle entry into an epidural space is a pivotal moment in cervical, thoracic, or lumbar epidural injections. Its significance cannot be overestimated. Regardless of how experienced you are, realizing that you are approaching an epidural space makes your heart beat faster. The safety of the procedure and its success rate depends upon proper and reliable identification of the ...

Improved agar gradient-plate technique.

MIC of antimicrobial compounds, it is essential to have uniform microbial growth on the agar medium; otherwise, it is difficult to determine a sharply defined area of growth inhibition. The conventional agar gradient-plate method has been found to be unsatisfactory for determining the MIC of chemotherapeutic compounds or antibiotics which inhibit filamentous fungi because the latter frequently ...

Cross spread pupil tracking technique

We present a fast and minimum delay algorithm for detecting the pupil center, called the cross spread tracking technique. This algorithm is meant for video eye trackers that estimate gaze direction from the position of the pupil center in the captured images. Contrary to other solutions, we do not try to make this technique robust to distractors such as reflections, distortions caused by glasse...

Perfecting peptide priming

Tumors can avoid immune destruction despite being invaded by tumor-specific CD8 ϩ T cells. On page 1257, Bronte et al. describe one way that prostate tumors subdue T cells— they make two enzymes that combine forces to cripple T cell signaling components. Tumors use a variety of tactics to avoid attack by cytotoxic CD8 ϩ T cells; one is to establish an inhospitable environment that inhibits T ce...